β-Glucuronidase of Rat Liver Lysosomes
نویسندگان
چکیده
منابع مشابه
Isolating Lysosomes from Rat Liver.
This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.
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Abalraet--The effect ofafiatoxin on the activities of 5 rat liver lysosomal enzymes (acid deoxyribonuclease, arylsulfatases A and B, ß-glucuronidase, ß-gluoosidase and ß-galactosidase) has been investigated In vivo and t vitro. Threehours afterthe administration of atjatoxin, activities of most of the enzymes increased significantly reaching maximal levels at 48 hr. The activity of acidDNase in...
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Amino acid efflux from highly purified rat liver lysosomes exposed to the methyl ester derivatives of leucine, methionine, tyrosine and cystine was examined. The lysosomal efflux of leucine, methionine and tyrosine was unaffected by the presence of MgATP, whereas cystine efflux was stimulated by MgATP. Exposure of lysosomes to 2 mM-MgATP resulted in lysosomal acidification and a 0.5 pH unit inc...
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Transport of D-glucose was studied in Percoll-gradient-purified rat liver lysosomes. D-Glucose uptake had a Km of 22 mM and a t1/2 of approx. 30 s. D-Fucose, 2-deoxyglucose and methyl alpha-glucoside were the most effective competitors for uptake of D-glucose, although D-galactose, D-mannose, D-xylose and L-fucose also appeared to compete for uptake. L-Glucose was a poor competitor for uptake. ...
متن کاملDigestive activity of lysosomes. I. The digestion of proteins by extracts of rat liver lysosomes.
The hydrolysis of acid-denatured human or bovine globin at 37” by extracts of highly purified rat liver lysosomes was most extensive between pH 4.4 and 5.6. After exhaustive digestion under these conditions, the ninhydrin-positive material released by enzymatic hydrolysis amounted to 70% of that released by acid hydrolysis. The main products of hydrolysis were free amino acids (42% of the total...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61921-0